Laboratory Technician

Process peripheral blood samples from SLE patients and at-risk individuals enrolled in the PRELUDE study, including PBMC isolation, cryopreservation, and biobanking Maintain and organise the PRELUDE study biobank, ensuring accurate sample tracking and storage logs Perform multiparameter flow cytometry to characterise regulatory T cell (Treg) phenotype and function, including panel preparation, sample acquisition, and data export Contribute to flow cytometry data analysis using FlowJo or equivalent software Conduct functional Treg suppression assays and other cell-based immunological assays as directed by the postdoctoral team Carry out molecular biology techniques including RNA extraction, cDNA synthesis, and gene expression analysis where required Maintain accurate and detailed laboratory records in line with GCP and institutional requirements To be able to follow departmental SOPs and prepare samples accurately To be able to use computer systems correctly, enter data accurately from transcription sheets or request forms To assist in the training and supervision of staff / students working in the Wincup Group as part of their induction. Ensure all research activity is conducted in accordance with relevant NHS and university research governance frameworks Attend and contribute to regular laboratory meetings and journal clubs Assist with ordering laboratory consumables and maintaining equipment logs Contribute to the preparation of data for presentations, grant reports, and manuscripts as directed Job description Job responsibilities Process peripheral blood samples from SLE patients and at-risk individuals enrolled in the PRELUDE study, including PBMC isolation, cryopreservation, and biobanking Maintain and organise the PRELUDE study biobank, ensuring accurate sample tracking and storage logs Perform multiparameter flow cytometry to characterise regulatory T cell (Treg) phenotype and function, including panel preparation, sample acquisition, and data export Contribute to flow cytometry data analysis using FlowJo or equivalent software Conduct functional Treg suppression assays and other cell-based immunological assays as directed by the postdoctoral team Carry out molecular biology techniques including RNA extraction, cDNA synthesis, and gene expression analysis where required Maintain accurate and detailed laboratory records in line with GCP and institutional requirements To be able to follow departmental SOPs and prepare samples accurately To be able to use computer systems correctly, enter data accurately from transcription sheets or request forms To assist in the training and supervision of staff / students working in the Wincup Group as part of their induction. Ensure all research activity is conducted in accordance with relevant NHS and university research governance frameworks Attend and contribute to regular laboratory meetings and journal clubs Assist with ordering laboratory consumables and maintaining equipment logs Contribute to the preparation of data for presentations, grant reports, and manuscripts as directed